Followed the content of total flavonoids in the crude extract and purification was determine by UV spectrophotometry. Finally, to study the total flavonoids toxicology. Total flavonoids from Fructus Canarii intragastrically administered mice in experimental medicine group, with the largest volume permitted and the thickest concentration. 其次采用紫外-可见分光光度法对粗提取物和纯化后的青果总黄酮进行含量测定。